This application addresses those who require a reliable tool for quantifying data from biological images, with the help of a modular and flexible approach. Competent application that will help users analyze microscope imagery much easier Novices are advised to refer to the in-depth documentation that can be accessed on the developer’s website, as this application will require advanced knowledge of biological phenology. After adding the preferred modules, users can easily switch between them by using the dedicated commands.Īdvanced users might be happy to know that CellProfiler also provides a plate viewer and features for visualizing the corresponding histogram, density plots or statistics. Accuracy and robustness of cell segmentation were assessed by using CellID IntercalatorIr to identify cell nuclei and pankeratin for cytoplasm (Fig. Users will be able to access an impressive array of predefined modules that offer tools for processing the loaded images, classifying objects, creating batch files or measuring correlations and overlaps. First, we used cellprofiler 2.2.0 (CP) to identify single cells through establishing nuclear and cellular boundaries 43. Easily process microscope imagery and identify phenotype characteristics with this useful application In order to improve the work efficiency and flexibility, the application allows users to add several predefined modules for creating their required workflow.
CELLPROFILER 2.2.0 UPDATE
One will be able to follow an intuitive analysis sequence, which provides an effective analysis flow: images, metadata, names / types and groups.Īfter loading the images that need to be analyzed, people will be able to analyze them, update their information and extract details from their corresponding metadata. The application comes packed with a clean interface that provides several modules where users can organize their workflow efficiently. The ImageXpress channel name refers to the text in the raw image file name identifying the acquired wavelength.
CELLPROFILER 2.2.0 SOFTWARE
Appealing interface that will allow one to add custom modules and use an intuitive analysis sequence The CellProfiler channel name refers to the name given by the software to each channel this nomenclature also applies to the naming of the extracted morphological features. It provides the means for extracting valuable biological data from images in a fast-paced environment, therefore increasing users’ workflow efficiency. CellProfiler was created in order to provide users with a way of collecting information about the imaged samples. Those who are involved into studying biological processes and use light / fluorescence microscopes for imaging cells and other biological samples could benefit from a tool for analyzing such images.
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